BinaxNOW® Avian Influenza Virus Type A Antigen Test
Kit is a single-use, rapid immunochromatographic assay for the
qualitative presumptive (first step) detection of avian influenza
type A viral antigens in oropharyngeal, tracheal and cloacal
samples from chickens, turkeys, and ducks.
Product Details
BinaxNOW Avian Influenza Virus Type A Antigen Test is the only
rapid membrane test that does not require a separate extraction
step for the test sample.
- Easy: one step assay - add sample and
close
- Rapid: produces results in 15
minutes
- Simple: no special equipment or
training required
- Flexible: can be used with
oropharyngeal, tracheal or cloacal swabs
BinaxNOW Avian Influenza Virus Type A Antigen Test is the first
rapid membrane test that has a negative functional control line for
each test.
- Environmentally friendly:
incineratable test and packaging
- Contained test: closed test
- Uncomplicated: nothing to mix,
nothing to spill
Claims
|
Species
|
Chickens, ducks, turkeys
|
|
Samples
|
Tracheal, oropharyngeal, cloacal swabs
|
|
Sensitivity
|
98.0% (99/101)
|
|
Specificity
|
99.5% (3,157/3,172)
|
|
Limit of detection
|
104 EID50 / ml |
|
Sampling
|
Individual samples; pools of 5 or 11
|
|
Days detection
|
3, 4, 5 days post-infection for both pools
|
| |
of 5 or 11 |
|
Transport Media
|
Amies Media, Brain Heart Infusion Broth, |
| |
Dulbecco’s Medium, Hank’s Balanced Salt |
| |
Solution, M4 Media, M4-RT Media, M5 |
| |
Media, Phosphate Buffer Saline, Stuart’s |
| |
Media, Tryptose Phosphate Broth, UTM-RT |
| |
Media, Veal Infusion Broth and Saline. |
|
Storage
|
2 – 30°C
|
|
Shelf life
|
Up to 24 months
|
|
Results
|
15 minutes |
BinaxNOW Avian Influenza Testing Procedure
Elute swab in 0.5-3.0 ml of an appropriate
liquid transport media by vigorously rotating the swab in the
liquid. If using a liquid sample, proceed to Test Procedure.
Remove device from the pouch just prior to testing
and lay flat on work area.
Fill pipette by firmly squeezing the top bulb
and then placing pipette tip into sample. Slowly
release bulb while tip is still in sample. This will pull liquid
into the pipette. Make sure there are
no air spaces in the lower part of the pipette.
See arrow on test device to find the
WHITE sample pad at the TOP of
the test strip. SLOWLY (drop by drop)
add entire
contents of the pipette (100 µl) to the
MIDDLE of this pad such that all of the sample
volume absorbs into this
pad. DO NOT add sample to the pink/purple colored
pad.
WARNING: INVALID RESULTS can occur when too
little sample is added to the test. Be sure that the lower part of
the transfer pipette is full and does not have any air spaces
before you add the sample to the sample pad. If there are air
spaces, put the sample back in the container by squeezing the top
bulb. Redraw the sample from the bottom of the container into the
pipette. Use a new pipette if needed.
Immediately peel off adhesive liner from the
test device. Close and securely seal the device. Read result in
window 15 minutes after closing the device. Note: When reading the
results, tilt the device to reduce glare on the result window if
necessary.
For full instructions for use, see package inserts:
BinaxNOW Avian Influenza
About the Test
The BinaxNOW test is an easy to use, rapid immunochromatographic
test (ICT) that detects influenza A nucleoproteins in
oropharyngeal, tracheal and cloacal swabs in chickens, turkeys, and
ducks. This ICT uses highly sensitive monoclonal antibodies to
detect influenza A nucleoprotein antigens. These antibodies and two
control antibodies are immobilized onto a membrane support to
construct a test strip. This test strip is mounted inside a
cardboard, book-shaped, hinged, test device. Results from the
one-step test are available in 15 minutes. Use of the BinaxNOW test
enables early and accurate identification of influenza A
nucleoproteins, thus facilitating the implementation of appropriate
and cost-effective action plans.
About Avian Influenza
Worldwide, there are many strains of avian influenza (AI) virus
that can cause varying amounts of clinical illness in
poultry.1, 2, 3 AI viruses can infect chickens, turkeys,
ducks, and other avian species, as well as humans. Migratory
waterfowl have proved to be a natural reservoir for the less
infectious strains of the disease known as low-pathogenic avian
influenza.4 AI viruses can be classified into
low-pathogenic (LPAI) and high-pathogenic (HPAI) based on the
severity of the illness they cause. HPAI is an extremely infectious
and fatal form of the disease that, once established, can spread
rapidly from flock to flock. However, some LPAI virus strains are
capable of mutating, under field conditions, into HPAI viruses. The
prominent subtypes of avian influenza A viruses include Influenza A
H5 and Influenza A H7.
Reference
- www.usda.gov/birdflu
- http://www.oie.int/
- www.fao.org/avianflu/en/index.html
- Field Manual of Wildlife Diseases: Birds. US Geological
Services, Chapter 22, p181-184.